Medicine
Reza Eghdam Zamiri; Saeid Charsouei
Abstract
Introduction: The exploration of biomarker profiles in breast carcinoma with nervous system metastasis represents a critical frontier in cancer research. This endeavor holds the potential to revolutionize diagnostic accuracy, prognostic precision, and therapeutic strategies for patients grappling with ...
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Introduction: The exploration of biomarker profiles in breast carcinoma with nervous system metastasis represents a critical frontier in cancer research. This endeavor holds the potential to revolutionize diagnostic accuracy, prognostic precision, and therapeutic strategies for patients grappling with this aggressive form of cancer. As research progresses, the integration of biomarker information into clinical practice may usher in a new era of personalized medicine, offering hope for improved outcomes and a better quality of life for those affected by breast carcinoma with nervous system metastasisMaterial and Methods: This retrospective study analyzed 150 breast carcinoma patients with nervous system metastasis. Clinical data were sourced from records, and tissue samples underwent immunohistochemistry and gene expression profiling. Statistical analyses, including survival curves, explored biomarker associations. A 75-patient validation cohort supported findings.Results: Microarray analysis of gene expression profiles identified distinct molecular signatures associated with nervous system metastasis. Pathway enrichment analysis revealed upregulation of genes associated with cell migration, angiogenesis, and neuroinflammation.Conclusion: Our exploration of biomarker profiles in breast carcinoma patients with nervous system metastasis has provided a nuanced perspective on the molecular intricacies of this formidable disease. The integration of clinical, pathological, and molecular data has facilitated a comprehensive understanding of the heterogeneity inherent in nervous system metastasis.
Medicine
Reza Eghdam Zamiri; Abdolreza Mehdinavaz Aghdam
Abstract
Introduction: moking increases the risk of postoperative pulmonary complications in patients receiving general anesthesia. During laparoscopic surgeries, the effects of pneumoperitoneum and carbon dioxide (CO2) insufflation may amplify these. Our goal was to compare the metabolic and blood gas analysis ...
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Introduction: moking increases the risk of postoperative pulmonary complications in patients receiving general anesthesia. During laparoscopic surgeries, the effects of pneumoperitoneum and carbon dioxide (CO2) insufflation may amplify these. Our goal was to compare the metabolic and blood gas analysis of patients who underwent laparoscopic surgeries while under general anesthesia compared to those who did not smoke.Material and Methods: After receiving approval from the institutional review board, the 60 patients undergoing laparoscopic cholecystectomy were split into two groups of 30 each: smokers and non-smokers. A arterial blood gas sampling was performed along with baseline hemodynamic parameters to evaluate and compare PCO2, pH, and bicarbonate (HCO3) values at different time intervals with respect to pneumo-peritoneum creation, between smokers and non-smokers.Results: Systolic blood pressure was higher at baseline in the smoker group, and oxygen saturation was noticeably lower. Smokers had significantly higher PCO2 and end-tidal CO2 at all timesConclusion: Between smokers and non-smokers, there is a clear distinction in baseline arterial blood gas characteristics. Smokers appear to be more susceptible to the metabolic effects of CO2 insufflation and elevated intraabdominal pressure.
Medicine
Reza Eghdam Zamiri; Farshad Mahdavi
Abstract
Introduction: More than 400 colorectal tissues, including colorectal adenomas and cancers, and a panel of six CRC cell lines were used to study the epigenetic regulation of miR-137. Material and Methods: We go over miR-137's epigenetic control and how it affects the development of colorectal cancer. ...
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Introduction: More than 400 colorectal tissues, including colorectal adenomas and cancers, and a panel of six CRC cell lines were used to study the epigenetic regulation of miR-137. Material and Methods: We go over miR-137's epigenetic control and how it affects the development of colorectal cancer. Six CRC cell lines, 50 colorectal tissues, 21 healthy individuals' normal colonic mucosa (N-N), 160 primary CRC tissues, and their corresponding normal mucosa (N-C), as well as 68 adenomas, were used to determine the methylation status of the miR-137 CpG island. We examined the expression of miR-137 using TaqMan RT-PCR and in situ hybridization. Results: MiR-137 was only expressed in colonic epithelial cells, which cover the entire colonic crypt, in normal colonic mucosa. However, none of the adenomatous and CRC samples exhibited miR-137 expression, supporting our finding that miR-137 is silenced in the majority of colonic neoplastic tissues. As a result of our discovery that CpG island methylation causes miR-137 to be epigenetically silenced in CRC, we then carried out functional studies to see if miR-137 had tumor-suppressive properties in vitro after transfecting CRC cell lines with miR-137 precursor. Conclusion: In conclusion, this study first explains that miR-137 acts as a tumor suppressor in the colon, is frequently silenced in CRC through promoter hypermethylation, and its restoration inhibits cell proliferation in vitro.
